Identification and quantification of lipid metabolites in cotton fibers: Reconciliation with metabolic pathway predictions from DNA databases.

Identification and quantification of lipid metabolites in cotton fibers: Reconciliation with metabolic pathway predictions from DNA databases.

The lipid composition of cotton (Gossypium hirsutum, L) fibers was determined. Fatty acid profiles revealed that linolenate and palmitate were the most abundant fatty acids present in fiber cells. Phosphatidylcholine was the predominant lipid class in fiber cells, while phosphatidylethanolamine, pho...

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Bibliographic Details
Main Author: Wanjie, Sylvia W.
Other Authors: Chapman, Kent D.
Format: Others
Language:English
Published: University of North Texas 2004
Subjects:
Cotton > Genetics.
Lipids > Metabolism.
lipid metabolism
cotton fibers
plant membranes
Online Access:https://digital.library.unt.edu/ark:/67531/metadc4474/
id ndltd-unt.edu-info-ark-67531-metadc4474
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spelling ndltd-unt.edu-info-ark-67531-metadc44742017-03-17T08:35:59Z Identification and quantification of lipid metabolites in cotton fibers: Reconciliation with metabolic pathway predictions from DNA databases. Wanjie, Sylvia W. Cotton -- Genetics. Lipids -- Metabolism. lipid metabolism cotton fibers plant membranes The lipid composition of cotton (Gossypium hirsutum, L) fibers was determined. Fatty acid profiles revealed that linolenate and palmitate were the most abundant fatty acids present in fiber cells. Phosphatidylcholine was the predominant lipid class in fiber cells, while phosphatidylethanolamine, phosphatidylinositol and digalactosyldiacylglycerol were also prevalent. An unusually high amount of phosphatidic acid was observed in frozen cotton fibers. Phospholipase D activity assays revealed that this enzyme readily hydrolyzed radioactive phosphatidylcholine into phosphatidic acid. A profile of expressed sequence tags (ESTs) for genes involved in lipid metabolism in cotton fibers was also obtained. This EST profile along with our lipid metabolite data was used to predict lipid metabolic pathways in cotton fiber cells. University of North Texas Chapman, Kent D. Pirtle, Robert M. Dickstein, Rebecca 2004-05 Thesis or Dissertation Text oclc: 55804232 https://digital.library.unt.edu/ark:/67531/metadc4474/ ark: ark:/67531/metadc4474 English Public Copyright Wanjie, Sylvia W. Copyright is held by the author, unless otherwise noted. All rights reserved.
collection NDLTD
language English
format Others
sources NDLTD
topic Cotton -- Genetics.
Lipids -- Metabolism.
lipid metabolism
cotton fibers
plant membranes
spellingShingle Cotton -- Genetics.
Lipids -- Metabolism.
lipid metabolism
cotton fibers
plant membranes
Wanjie, Sylvia W.
Identification and quantification of lipid metabolites in cotton fibers: Reconciliation with metabolic pathway predictions from DNA databases.
description The lipid composition of cotton (Gossypium hirsutum, L) fibers was determined. Fatty acid profiles revealed that linolenate and palmitate were the most abundant fatty acids present in fiber cells. Phosphatidylcholine was the predominant lipid class in fiber cells, while phosphatidylethanolamine, phosphatidylinositol and digalactosyldiacylglycerol were also prevalent. An unusually high amount of phosphatidic acid was observed in frozen cotton fibers. Phospholipase D activity assays revealed that this enzyme readily hydrolyzed radioactive phosphatidylcholine into phosphatidic acid. A profile of expressed sequence tags (ESTs) for genes involved in lipid metabolism in cotton fibers was also obtained. This EST profile along with our lipid metabolite data was used to predict lipid metabolic pathways in cotton fiber cells.
author2 Chapman, Kent D.
author_facet Chapman, Kent D.
Wanjie, Sylvia W.
author Wanjie, Sylvia W.
author_sort Wanjie, Sylvia W.
title Identification and quantification of lipid metabolites in cotton fibers: Reconciliation with metabolic pathway predictions from DNA databases.
title_short Identification and quantification of lipid metabolites in cotton fibers: Reconciliation with metabolic pathway predictions from DNA databases.
title_full Identification and quantification of lipid metabolites in cotton fibers: Reconciliation with metabolic pathway predictions from DNA databases.
title_fullStr Identification and quantification of lipid metabolites in cotton fibers: Reconciliation with metabolic pathway predictions from DNA databases.
title_full_unstemmed Identification and quantification of lipid metabolites in cotton fibers: Reconciliation with metabolic pathway predictions from DNA databases.
title_sort identification and quantification of lipid metabolites in cotton fibers: reconciliation with metabolic pathway predictions from dna databases.
publisher University of North Texas
publishDate 2004
url https://digital.library.unt.edu/ark:/67531/metadc4474/
work_keys_str_mv AT wanjiesylviaw identificationandquantificationoflipidmetabolitesincottonfibersreconciliationwithmetabolicpathwaypredictionsfromdnadatabases
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