Use of tRNA Gene Probes to Identify Polymorphic Loci in the Bovine Genome

• Main Author: Shariat, Parvaneh
• Other Authors: Benjamin, Robert C.
• Format: Others
• Language: English
• Published: University of North Texas 1998
• Subjects: tRNA gene probes; polymorphic loci; bovine genomes; Transfer RNA.; Genomes.
• Online Access: https://digital.library.unt.edu/ark:/67531/metadc278338/

A 30-mer oligonucleotide probe encoding the "A box" and anticodon loop regions of a human glycine tRNA gene was used to isolate a 581bp DNA fragment from a bovine genomic DNA library. Although the cross-hybridizing segment of DNA was found not to encode any tRNA gene or pseudogene, a region with homology to the "C-element" of the "BOV-tA" type Alulike artiodactyl retroposons was identified. This cross-hybridization was determined to be the result of conserved RNA polymerase III promoter elements in the probe portion of the tRNA gene and these repetitive elements. A microsatellite repeat (TC) was also found associated with this element. Future screening for bovine tRNA genes will require the use of a) longer probes and higher stringency hybridization conditions or b) the simultaneous screening with probes from the 5' and 3' ends of the gene which avoid the conserved Pol III promoter boxes.