Rhizosphere biotransformation of selected polychlorinated biphenyl (PCB) congeners by switchgrass and poplar

• Main Author: Meggo, Richard Edward
• Other Authors: Schnoor, Jerald L.
• Format: Others
• Language: English
• Published: University of Iowa 2012
• Subjects: Biotransformation; PCB; Poplar; Rhizosphere; Switchgrass; Civil and Environmental Engineering
• Online Access: https://ir.uiowa.edu/etd/3499; https://ir.uiowa.edu/cgi/viewcontent.cgi?article=3500&context=etd

Selected PCB congeners (PCB 52, 77, and 153) singly and in mixtures were spiked and aged in soil microcosms and subsequently planted with switchgrass (Panicum virgatum) or poplar (Populus deltoids x nigra DN34). The planted reactors showed significantly greater reductions in PCB parent compounds when compared to unplanted systems after 32 weeks, both in single congener exposures and when all three congeners were present in a mixture. There was evidence of reductive dechlorination in both planted and unplanted systems, but higher concentrations of transformation products were observed in the planted systems than the unplanted. Although planted systems resulted in greater biotransformation, this improvement in PCB-reduction was not the result of plant uptake but rather was due to transformations occurring in the root rhizosphere. Parent PCB congeners were transformed by reductive dechlorination resulting in successively less chlorinated PCB congeners. These dechlorination products accounted for approximately all of the molar mass of parent compound lost. Based on the transformation products, reductive dechlorination pathways are proposed for rhizospheric biotransformation of PCB 52, 77, and 153. Results suggest that PCB 52 transformation proceeds through PCBs 18 and 9 down to monochlorinated PCB 1. Biotransformation of PCB 77 occurs through the intermediaries PCB 35 and 37. The pathway for the rhizospheric transformation of PCB 153 is through PCB 101 and PCB 99. This study provides insight into rhizosphere biotransformation pathways for reductive dechlorination in marginally aerobic,intermittently flooded soil as evidenced by a mass balance on transformation products. Despite the marginally aerobic conditions it is likely that highly reduced microzones existed in the soil particles during flooding and provided the opportunity for reductive dechlorination. In these experiments, planted microcosms with fully developed roots and rhizospheres showed significant reductive dechlorination and greater biotransformation than unplanted reactors. In addition, planted systems that were intermittently flooded had greater transformation of the parent PCB compounds than systems that were not. A poplar planted system resulted in the complete removal of 26 of the 29 PCB congeners detected in a commercial garden soil, while the unplanted soil only had 2 congeners completely removed after 96 days. In addition, the most recalcitrant congener, PCB 52, only decreased by 0.1% in the unplanted reactors while declining by 22.3% in the planted system. There was also greater removal of a PCB 77 spike in the planted system when compared to the unplanted system, 17.2% in the planted system versus 2.8% in the unplanted system. The results suggest that phytoremediation may be an effective tool in cleaning commercially available garden soils that are lightly contaminated with PCBs.