Effect of adenosine and lidocaine on cardiac functional and metabolic recovery after global ischemia and reperfusion

This study investigated if exogenous adenosine (ADO) improves recovery of cardiac function during repetfusion (RPF) after global ischemia (ISC), and if lidocaine is required for. ADO-mediated cardioprotection during reperfusion. Isolated rabbit hearts, retrogradely perfused with erythrocyte-enriched...

Full description

Bibliographic Details
Main Author: Vos, Lynette C.
Format: Others
Published: Scholarly Commons 1994
Subjects:
Online Access:https://scholarlycommons.pacific.edu/uop_etds/2260
https://scholarlycommons.pacific.edu/cgi/viewcontent.cgi?article=3259&context=uop_etds
id ndltd-pacific.edu-oai-scholarlycommons.pacific.edu-uop_etds-3259
record_format oai_dc
spelling ndltd-pacific.edu-oai-scholarlycommons.pacific.edu-uop_etds-32592021-10-05T05:13:17Z Effect of adenosine and lidocaine on cardiac functional and metabolic recovery after global ischemia and reperfusion Vos, Lynette C. This study investigated if exogenous adenosine (ADO) improves recovery of cardiac function during repetfusion (RPF) after global ischemia (ISC), and if lidocaine is required for. ADO-mediated cardioprotection during reperfusion. Isolated rabbit hearts, retrogradely perfused with erythrocyte-enriched Krebs-Henseleit buffer at constant left ventricular (LV) volume and physiologic flow rates, were subjected to 20 min. of global no-flow ischemia, and reperfused at the same rate as before ischemia. Hearts received one of the following treatments: 1) control (CON; no drug treatment), 2) adenosine (ADO; 200J.1M before and after ISC), or 3) adenosine+lidocaine (NL; 200 JlM ADO before and after ISC, 1 J.Lg/ml/min LIDO during first 20 min. of RPF). Myocardial function (e.g., using developed LV pressure, DP) declined as expected during no-flow ischemia and gradually returned during reperfusion. Functional recovery in ADO and NL groups were significantly improved from CON during early RPF (p<0.05 at 2 min RPF), but not at later RPF times(> 10 min). Differences did not exist between ADO and NL groups at any RPF time except at 10 min. RPF. Additionally, myocardial ATP content was measured before ischemia, after ischemia, and after 10 and 30 min of reperfusion. ATP content decreased significantly during ischemia; ADO hearts showed a increased repletion (85% of pre-ischemia level) of ATP at 30 min. of reperfusionas compared to CON (60%). These data suggest that ADO alone improves cardiac functional recovery during early repetfusion; LIDO does not appear to be required for ADO-mediated cardioprotection. ADO and LIDO do not improve cardiac function, however, ADO appears to improve myocardial ATP repletion at later RPF times in this blood-perfused rabbit model of global myocardial ISC/RPF. 1994-01-01T08:00:00Z text application/pdf https://scholarlycommons.pacific.edu/uop_etds/2260 https://scholarlycommons.pacific.edu/cgi/viewcontent.cgi?article=3259&amp;context=uop_etds University of the Pacific Theses and Dissertations Scholarly Commons Heart Physiology Reperfusion (Physiology) Myocardial reperfusion Adenosine Lidocaine Medicine and Health Sciences
collection NDLTD
format Others
sources NDLTD
topic Heart Physiology
Reperfusion (Physiology)
Myocardial reperfusion
Adenosine
Lidocaine
Medicine and Health Sciences
spellingShingle Heart Physiology
Reperfusion (Physiology)
Myocardial reperfusion
Adenosine
Lidocaine
Medicine and Health Sciences
Vos, Lynette C.
Effect of adenosine and lidocaine on cardiac functional and metabolic recovery after global ischemia and reperfusion
description This study investigated if exogenous adenosine (ADO) improves recovery of cardiac function during repetfusion (RPF) after global ischemia (ISC), and if lidocaine is required for. ADO-mediated cardioprotection during reperfusion. Isolated rabbit hearts, retrogradely perfused with erythrocyte-enriched Krebs-Henseleit buffer at constant left ventricular (LV) volume and physiologic flow rates, were subjected to 20 min. of global no-flow ischemia, and reperfused at the same rate as before ischemia. Hearts received one of the following treatments: 1) control (CON; no drug treatment), 2) adenosine (ADO; 200J.1M before and after ISC), or 3) adenosine+lidocaine (NL; 200 JlM ADO before and after ISC, 1 J.Lg/ml/min LIDO during first 20 min. of RPF). Myocardial function (e.g., using developed LV pressure, DP) declined as expected during no-flow ischemia and gradually returned during reperfusion. Functional recovery in ADO and NL groups were significantly improved from CON during early RPF (p<0.05 at 2 min RPF), but not at later RPF times(> 10 min). Differences did not exist between ADO and NL groups at any RPF time except at 10 min. RPF. Additionally, myocardial ATP content was measured before ischemia, after ischemia, and after 10 and 30 min of reperfusion. ATP content decreased significantly during ischemia; ADO hearts showed a increased repletion (85% of pre-ischemia level) of ATP at 30 min. of reperfusionas compared to CON (60%). These data suggest that ADO alone improves cardiac functional recovery during early repetfusion; LIDO does not appear to be required for ADO-mediated cardioprotection. ADO and LIDO do not improve cardiac function, however, ADO appears to improve myocardial ATP repletion at later RPF times in this blood-perfused rabbit model of global myocardial ISC/RPF.
author Vos, Lynette C.
author_facet Vos, Lynette C.
author_sort Vos, Lynette C.
title Effect of adenosine and lidocaine on cardiac functional and metabolic recovery after global ischemia and reperfusion
title_short Effect of adenosine and lidocaine on cardiac functional and metabolic recovery after global ischemia and reperfusion
title_full Effect of adenosine and lidocaine on cardiac functional and metabolic recovery after global ischemia and reperfusion
title_fullStr Effect of adenosine and lidocaine on cardiac functional and metabolic recovery after global ischemia and reperfusion
title_full_unstemmed Effect of adenosine and lidocaine on cardiac functional and metabolic recovery after global ischemia and reperfusion
title_sort effect of adenosine and lidocaine on cardiac functional and metabolic recovery after global ischemia and reperfusion
publisher Scholarly Commons
publishDate 1994
url https://scholarlycommons.pacific.edu/uop_etds/2260
https://scholarlycommons.pacific.edu/cgi/viewcontent.cgi?article=3259&amp;context=uop_etds
work_keys_str_mv AT voslynettec effectofadenosineandlidocaineoncardiacfunctionalandmetabolicrecoveryafterglobalischemiaandreperfusion
_version_ 1719487614094934016