Investigation of the expression of DMC1, a meiotic gene, in trichomonas vaginalis
T. vaginalis is a protozoan parasite without an observed sexual stage in its life cycle. However, T. vaginalis has genes, such as Dmc1, known to be involved in meiosis in other organisms. In order to look at the expression of these genes in T. vaginalis, RT-PCR was done using purified mRNA. It shows...
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Scholarly Commons
2007
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ndltd-pacific.edu-oai-scholarlycommons.pacific.edu-uop_etds-16632021-10-05T05:12:31Z Investigation of the expression of DMC1, a meiotic gene, in trichomonas vaginalis Fullerton, Donna Lynn T. vaginalis is a protozoan parasite without an observed sexual stage in its life cycle. However, T. vaginalis has genes, such as Dmc1, known to be involved in meiosis in other organisms. In order to look at the expression of these genes in T. vaginalis, RT-PCR was done using purified mRNA. It shows that Dmc1 is expressed in both normal and drug treated cells. However, relative levels are unclear. Localization studies were done in T. vaginalis using immunofluorescence against Dmc1 protein with an HA tag. These studies showed that recombinant Dmc1 remained in the cytoplasm upon treatment with DNA damaging drugs. Additionally, T.vaginalis Dmc1 protein was expressed and purified from E.coli to have polyclonal antibodies made to use in further immunofluorescence studies. 2007-01-01T08:00:00Z text application/pdf https://scholarlycommons.pacific.edu/uop_etds/664 https://scholarlycommons.pacific.edu/cgi/viewcontent.cgi?article=1663&context=uop_etds University of the Pacific Theses and Dissertations Scholarly Commons Trichomonas vaginalis Meiosis Gene expression Life Sciences |
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Others
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NDLTD |
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Trichomonas vaginalis Meiosis Gene expression Life Sciences |
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Trichomonas vaginalis Meiosis Gene expression Life Sciences Fullerton, Donna Lynn Investigation of the expression of DMC1, a meiotic gene, in trichomonas vaginalis |
| description |
T. vaginalis is a protozoan parasite without an observed sexual stage in its life cycle. However, T. vaginalis has genes, such as Dmc1, known to be involved in meiosis in other organisms. In order to look at the expression of these genes in T. vaginalis, RT-PCR was done using purified mRNA. It shows that Dmc1 is expressed in both normal and drug treated cells. However, relative levels are unclear. Localization studies were done in T. vaginalis using immunofluorescence against Dmc1 protein with an HA tag. These studies showed that recombinant Dmc1 remained in the cytoplasm upon treatment with DNA damaging drugs. Additionally, T.vaginalis Dmc1 protein was expressed and purified from E.coli to have polyclonal antibodies made to use in further immunofluorescence studies. |
| author |
Fullerton, Donna Lynn |
| author_facet |
Fullerton, Donna Lynn |
| author_sort |
Fullerton, Donna Lynn |
| title |
Investigation of the expression of DMC1, a meiotic gene, in trichomonas vaginalis |
| title_short |
Investigation of the expression of DMC1, a meiotic gene, in trichomonas vaginalis |
| title_full |
Investigation of the expression of DMC1, a meiotic gene, in trichomonas vaginalis |
| title_fullStr |
Investigation of the expression of DMC1, a meiotic gene, in trichomonas vaginalis |
| title_full_unstemmed |
Investigation of the expression of DMC1, a meiotic gene, in trichomonas vaginalis |
| title_sort |
investigation of the expression of dmc1, a meiotic gene, in trichomonas vaginalis |
| publisher |
Scholarly Commons |
| publishDate |
2007 |
| url |
https://scholarlycommons.pacific.edu/uop_etds/664 https://scholarlycommons.pacific.edu/cgi/viewcontent.cgi?article=1663&context=uop_etds |
| work_keys_str_mv |
AT fullertondonnalynn investigationoftheexpressionofdmc1ameioticgeneintrichomonasvaginalis |
| _version_ |
1719487157207302144 |