Studium struktury a metabolismu spermií savců a ryb ve vztahu k procesu reprodukce

Studium struktury a metabolismu spermií savců a ryb ve vztahu k procesu reprodukce

4. CONCLUSION Duringthe studyof teleosteanand chondrosteanfish spermatozoa,a new complex protocol,optimizedforimmunofluorescencestainingofantigenson carpandsturgeon spermatozoa,was developed lt was derivedfrom the protocolused for the mammalianmodel;however,theprotocolrespectsthesimpleandbreakablest...

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Main Author: Koubek, Pavel
Other Authors: Pěknicová, Jana
Format: Doctoral Thesis
Language:Czech
Published: 2009
Online Access:http://www.nusl.cz/ntk/nusl-454044
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spelling ndltd-nusl.cz-oai-invenio.nusl.cz-4540442021-11-29T05:23:23Z Studium struktury a metabolismu spermií savců a ryb ve vztahu k procesu reprodukce The study of structure and metabolism of mammalian and fish sperm in relation to the process of reproduction Koubek, Pavel Pěknicová, Jana Komrsková, Kateřina Flajšhans, Martin 4. CONCLUSION Duringthe studyof teleosteanand chondrosteanfish spermatozoa,a new complex protocol,optimizedforimmunofluorescencestainingofantigenson carpandsturgeon spermatozoa,was developed lt was derivedfrom the protocolused for the mammalianmodel;however,theprotocolrespectsthesimpleandbreakablestructure offishsperm.Inthisway,possibledamagetospermatozoaduringsamplepreparation is minimizedtogetherwithgenerationof nonspecificartefactswhichcouldinfluence the results.Therefore,it is crucialto createandfollowa suitableprotocolforsemen handlingand immunofluorescencestainingnotonlyforevaluationof semenquality butalsoforexperimentalstudreswhenthereproducibilityofthemethodis essential. In the study of energy metabolismon the model of demembranatedcarp spermatozoa,we provedtheexistenceof PCr shuttleand showedthe localizationof creatinekinaseisoenzymesalongthecarpspermflagellumbytheaidof monoclonal antibody.Moreover,withthe contributionof experimentsusinginhibitionof creatine kinase,we demonstratedthe possibilityof initiationand maintenanceof cAMP- independentflagellarmovementof demembranatedcarpspermatozoaonlyby the componentsofthePCr shuttle The studyof chondrosteanfishwas focusedon thefinestructureand morphologyof sterletspermatozoa,which was examinedby scanningand transmisionelectron... 2009 info:eu-repo/semantics/doctoralThesis http://www.nusl.cz/ntk/nusl-454044 cze info:eu-repo/semantics/restrictedAccess
collection NDLTD
language Czech
format Doctoral Thesis
sources NDLTD
description 4. CONCLUSION Duringthe studyof teleosteanand chondrosteanfish spermatozoa,a new complex protocol,optimizedforimmunofluorescencestainingofantigenson carpandsturgeon spermatozoa,was developed lt was derivedfrom the protocolused for the mammalianmodel;however,theprotocolrespectsthesimpleandbreakablestructure offishsperm.Inthisway,possibledamagetospermatozoaduringsamplepreparation is minimizedtogetherwithgenerationof nonspecificartefactswhichcouldinfluence the results.Therefore,it is crucialto createandfollowa suitableprotocolforsemen handlingand immunofluorescencestainingnotonlyforevaluationof semenquality butalsoforexperimentalstudreswhenthereproducibilityofthemethodis essential. In the study of energy metabolismon the model of demembranatedcarp spermatozoa,we provedtheexistenceof PCr shuttleand showedthe localizationof creatinekinaseisoenzymesalongthecarpspermflagellumbytheaidof monoclonal antibody.Moreover,withthe contributionof experimentsusinginhibitionof creatine kinase,we demonstratedthe possibilityof initiationand maintenanceof cAMP- independentflagellarmovementof demembranatedcarpspermatozoaonlyby the componentsofthePCr shuttle The studyof chondrosteanfishwas focusedon thefinestructureand morphologyof sterletspermatozoa,which was examinedby scanningand transmisionelectron...
author2 Pěknicová, Jana
author_facet Pěknicová, Jana
Koubek, Pavel
author Koubek, Pavel
spellingShingle Koubek, Pavel
Studium struktury a metabolismu spermií savců a ryb ve vztahu k procesu reprodukce
author_sort Koubek, Pavel
title Studium struktury a metabolismu spermií savců a ryb ve vztahu k procesu reprodukce
title_short Studium struktury a metabolismu spermií savců a ryb ve vztahu k procesu reprodukce
title_full Studium struktury a metabolismu spermií savců a ryb ve vztahu k procesu reprodukce
title_fullStr Studium struktury a metabolismu spermií savců a ryb ve vztahu k procesu reprodukce
title_full_unstemmed Studium struktury a metabolismu spermií savců a ryb ve vztahu k procesu reprodukce
title_sort studium struktury a metabolismu spermií savců a ryb ve vztahu k procesu reprodukce
publishDate 2009
url http://www.nusl.cz/ntk/nusl-454044
work_keys_str_mv AT koubekpavel studiumstrukturyametabolismuspermiisavcuarybvevztahukprocesureprodukce
AT koubekpavel thestudyofstructureandmetabolismofmammalianandfishsperminrelationtotheprocessofreproduction
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