Studium translačních iniciačních faktorů proteinové rodiny eIF4E ve vztahu k akutním lymfoblastickým leukemiím a k ontogenezi Tribolium castaneum

• Main Author: Mrvová, Silvia
• Other Authors: Mašek, Tomáš
• Format: Doctoral Thesis
• Language: Slovak
• Published: 2021
• Online Access: http://www.nusl.cz/ntk/nusl-451767

Translation initiation is one of the key moment in the cell at which many translation initiation factors participate. This step must be carefully regulated not to waste energy. Also excess or lack of many proteins can lead to different diseases. Translation initiation factor eIF4E is important mainly because of its ability to bind a cap and eIF4G and therefore its role in translation initiation. There are three isoforms in human cells - eIF4E1, eIF4E2, eIF4E3. eIF4E1 has been the most studied isoform; however the attention nowadays is drawn to the other two and their functions in cells as well. Upregulation of eIF4E1 is connected with different types of tumors or malignancies. The role of eIF4E isoforms comes to the fore, there has been a research in many model organisms, for example D. melanogaster or C. elegans where the individual isoforms have a role in maturation of oocytes, in forming body axes etc. I focused on all three isoforms of eIF4E, their 3′-UTR regions in lymphoblastic leukemic cells and the utilization of polyadenylation signals of these transcripts because there were shown several types of post-transcriptional regulations, for example stabilization of transcripts by HuR protein. I characterized 3′-UTR regions of eIF4E1, eIF4E2 and eIF4E3 transcripts and confirmed utilization of...