Význam trombofilních a imunologických faktorů v lidské reprodukci

• Main Author: Šubrt, Ivan
• Other Authors: Ulčová-Gallová, Zdeňka
• Format: Doctoral Thesis
• Language: Czech
• Published: 2013
• Online Access: http://www.nusl.cz/ntk/nusl-450855

The aim of presented study was to compare frequencies of eight antiphospholipid antibodies (aPL) in serum and assorted genetic thrombophilic factors and their mutual relation in patients with recurrent pregnancy loss (RPL) and controls. Enzyme-linked immunosorbent assay was used for detection of aPL against phosphatidyl-L-serine, phosphatidylethanolamine, phosphatidylinositol, phosphatidyl- DL-glycerol, phosphatidic acid, annexin V, cardiolipin, and beta2-GPI. Thrombophilic mutations factor V Leiden (F5 G1691A), F II G20210A, and MTHFR C677T and A1298C variants were determined using a melting curve analysis of the PCR amplification product detected by the fluorescence resonance energy transfer (FRET). PAI1 (-675)4G/5G, PROZ intron F G79A, PROZ A(-13)G and PROZ R255H variants were determined using standard PCR-RFLP method. Genotypes distribution and allelic frequencies were calculated. Correlation between aPL and thrombophilic factors was tested by chi-square and Fisher exact test. Our results showed significantly increased prevalence of aPL against phosphatidylinositol (17 - 19.6 % dependent on number of spontaneous miscarriages) and against phosphatidyl-L-serine (18-25 %). aPL in IgG prevailed. In 96 % of studied group we found at least one risk factor (either aPL positivity or thrombophilic factor). Both...