Využití LC-MS v bioanalýze antracyklinů a potenciálních kardioprotektiv

• Main Author: Bavlovič Piskáčková, Hana
• Other Authors: Štěrbová, Petra
• Format: Doctoral Thesis
• Language: Czech
• Published: 2021
• Online Access: http://www.nusl.cz/ntk/nusl-446323

Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Mgr. Hana Bavlovič Piskáčková Supervisor - specialist: doc. PharmDr. Radim Kučera Ph.D. Supervisor: doc. PharmDr. Petra Štěrbová Ph.D. Title: Ultilization of LC-MS in bioanalysis of anthracyclines and potential cardioprotective compounds Coupling of liquid chromatography to mass spectrometry (LC-MS) represents a near-ideal analytical tool for drug bioanalysis. This instrumentation allows identification and quantification with high selectivity and sensitivity, and provides information on the structure of tested compounds. An integral part of bioanalytical methods is the sample treatment prior to analysis, which should include isolation of the analytes, remove ballast from the matrix and ideally preconcentrate the sample. The sample treatment should be effective, quick, simple and repeatable. Current trends also emphasis process automation, high throughput and low consumption of organic solvent, which is in accordance with green chemistry. Dexrazoxane (DEX) is the only approved, clinically used drug, preventing chronic cumulative cardiotoxicity caused by anthracycline (ANT) therapy - commonly used anticancer drugs. The development of novel and more effective...