| Summary: | Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Nikola Rychla Supervisor: Doc. Ing. Petra Matoušková, Ph.D. Title of diploma thesis: Plasmid constuction for expression of selected reductase from Haemonchus contortus Barber's pole worm (Haemonchus contortus) is a pathogenic, gastroinstetinal, blood- sucking nematode. Drug resistance in H. contortus, and the associated haemonchosis of small ruminants such as sheep and goats, remain a major problem, and their biological and economic impacts are extensive. Given the fact that anthelmintics are still the gold standard of treatment, their reaserch is crucial, not only for farm animals but also for people. Carbonyl-reducing enzymes, such as aldoketoreductases and short-chain dehydrogenases/reductases, belong to the enzymes of the first phase of xenobiotics biotransformation and thus participate in drug metabolism and their increased elimination. Increased elimination leads to decreased toxicity in H. contortus and reduced efficacy of drugs in ruminants. Research of these enzymes can help tackle with drug resistance in H. contortus and contribute to the development of new drugs in the future. The aim of this diploma thesis is based on the construction of plasmid, which will be used for...
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