Organely mitochondriálního původu u diplomonád
Order Diplomonadida includes parasitic and free-living species that adapted to the oxygen-poor environment. They possess reduced form of mitochondria (hydrogenosome or mitosome). These organelles lack Krebs cycle and membrane electron-transport chain. ATP synthesis by oxidative phosphorylation and o...
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| Format: | Dissertation |
| Language: | English |
| Published: |
2019
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| Online Access: | http://www.nusl.cz/ntk/nusl-392923 |
| Summary: | Order Diplomonadida includes parasitic and free-living species that adapted to the oxygen-poor environment. They possess reduced form of mitochondria (hydrogenosome or mitosome). These organelles lack Krebs cycle and membrane electron-transport chain. ATP synthesis by oxidative phosphorylation and other mitochondrial metabolic pathways are modified or entirely absent. Main difference between hydrogenosome and mitosome is synthesis of hydrogen using the enzyme hydrogenase and ATP synthesis by substrate level phosphorylation in hydrogenosomes that are absent in mitosomes. The most studied diplomonads are a human pathogen Giardia intestinalis possessing the mitosomes and a salmon parasite Spironucleus salmonicida with hydrogenosomes. This thesis was focused on determining the type of mitochondrial organelles in angelfish parasite Spironucleus vortens and free living Hexamita sp. It has not been described whether they possess the hydrogenosomes or the mitosomes so far. In both protists transmission electron microscopy revealed presence of double membrane vesicles, possibly their mitochondrial organelles. Homologous S. vortens anti- hydrogenase and anti-HydE antibodies were produced and tested in order to determine their cellular localization. Using the western blot analysis and immunofluorescence... |
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