Vliv flubendazolu a mebendazolu na epiteliálně-mezenchymální tranzici.

• Main Author: Hochmalová, Martina
• Other Authors: Skálová, Lenka
• Format: Dissertation
• Language: Slovak
• Published: 2018
• Online Access: http://www.nusl.cz/ntk/nusl-388721

Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Martina Hochmalová Supervisor: prof. RNDr. Lenka Skálová, Ph.D. Consultant: RNDr. Věra Králová, Ph.D. Title of diploma thesis: The effect of flubendazole and mebendazole on the epithelial- mesenchymal transition. Epithelial-mesenchymal transition (EMT) is a process in which a non-motile epithelial cell switches to motile mesenchymal phenotype. This phenomenon is typical for multiple biological processes including cancer metastases. It could be induced by transforming growth factor-β (TGF-β) which is produced by cancer cells and which regulates its key transcription factors including SNAIL, ZEB and TWIST. EMT involves a series of defined events mediated by microtubules, including changes in cell shape and migration into the surrounding tissue. Based on these findings we suppose that the inhibition of tubulin polymerization could prevent the EMT process therefore cell migration and also cancer metastases. In our experiments, the effect of anthelminitic drugs flubendazole (FLU) and mebendazole (MBZ) on TGF-β-induced EMT in dysplastic oral keratinocytes was tested in vitro. Expression of molecules involved in EMT process was examined on microRNA and mRNA level using RT-PCR. The protein level of EMT...