Vliv seskviterpenů na detoxikační enzymy v jaterních řezech

• Main Author: Horáčková, Hana
• Other Authors: Szotáková, Barbora
• Format: Dissertation
• Language: Czech
• Published: 2018
• Online Access: http://www.nusl.cz/ntk/nusl-382785

Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Hana Horáčková Supervisor: prof. Ing. Barbora Szotáková, Ph.D. Title of diploma thesis: Effect of sesquiterpenes on detoxifying enzymes in liver slices Sesquiterpenes are secondary metabolites produced by higher plants, bacteria and fungi. They ensure the survival and competitiveness of plants. Sesquiterpenes are composed of three isoprene units. They possess anti-inflammatory, antimalarial, antibacterial, antiviral, antioxidant, analgesic, antifungal and anticancer activity. Sesquiterpenes, the main components of plant essential oils, have been used in folk medicine and as spices for years. The aim of this diploma thesis was to find out the effect of three structurally related sesquiterpenes α-humulene (HUM), β-caryophyllene (CAR), and β-caryophyllene oxide (CAO) on the activities of the main drug-metabolizing enzymes. Precision-cut tissue slices from the rat liver (Rattus norvegicus) were chosen as model system. Thickness of the slices was 200-250 μm and diameter 8 mm. Liver slices were pre-incubated for 30 minutes and then incubated for 8 and 24 hours. Samples of liver slices and incubation medium were taken upon replacing the medium after pre-incubation (time 0 hours) and then after 8 and 24...