Nesilikagelové materiály v analýze léčiv IV.

• Main Author: Ženíšková, Tereza
• Other Authors: Kučera, Radim
• Format: Dissertation
• Language: Czech
• Published: 2018
• Online Access: http://www.nusl.cz/ntk/nusl-380447

Non-silica based materials in drug analysis IV. Diploma thesis Tereza Ženíšková Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Heyrovského 1203, Hradec Králové. Sample preparation is an important step before the analysis itself. It enables removal of the ballast components, which could interfere with an analyte or even disable the analysis. Solid phase extraction (SPE) belongs among the most popular sample preparation techniques. Silica is the most often used sorbent for SPE, however new, more durable sorbents with different selectivity have appeared. This thesis focuses on employment of zirconium dioxide and its ability to retain analytes having Lewis base character via ligand exchange. We tested to use zirconium dioxide in extraction of indomethacin as a Lewis base from suppositories. The influence of conditioning solvents with different polarity and extractions solvents was investigated. 10% acetonitrile in methylene chloride was found to be the best solvent for conditioning of zirconia. Methanolic ammonia solution was used as eluent. After some experiments the concentration and the volume of the eluent was set at 0.25M NH3 in MeOH and 3 mL. Diclofenac was used as the internal standard. The recovery for sorbent after...