Vliv humulenu a karyofylenoxidu na účinek 5-fluorouracilu ve střevních nádorových buňkách

• Main Author: Šadibolová, Michaela
• Other Authors: Skálová, Lenka
• Format: Dissertation
• Language: Czech
• Published: 2017
• Online Access: http://www.nusl.cz/ntk/nusl-368151

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Michaela Šadibolová Supervisor: Prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The effect of humulene and caryophyllene oxide on the efficacy of 5-fluorouracil in colon cancer cells 5-Fluorouracil (5-FU) represents a standard chemotherapeutic agent used in the treatment of several types of cancer diseases including the treatment of colorectal cancer. However, the clinical use of 5-FU is limited due to frequent chemoresistance of tumour cells. Sesquiterpenes are plant secondary metabolites that form a major part of plant essential oils. They have been found to possess a considerable antitumour activity and the ability to enhance the efficacy of some traditional chemotherapeutic agents. The aim of this study was to find out whether sesquiterpenes α-humulene (HUM) and caryophyllene oxide (CAO) influence the antiproliferative effect of 5-FU in human colorectal adenocarcinoma cell lines Caco-2 and SW-620 and to test the effect of 5-FU, CAO and their combination on the activity of initiator caspases 8 and 9 and effector caspase 3/7 in Caco-2 cell line. CAO showed strong antiproliferative effect in both cancer cell lines whereas HUM showed minimal effect on cell proliferation. In...