Diverzita nekultivovatelných a obtížně kultivovatelných zástupců čeledi Psalteriomonadidae (Excavata: Heterolobosea)

• Main Author: Uhrová, Kristýna
• Other Authors: Čepička, Ivan
• Format: Dissertation
• Language: Czech
• Published: 2017
• Online Access: http://www.nusl.cz/ntk/nusl-368059

Heterolobosea is a group of amoeboflagellates, amoebae, and flagellates belonging to the supergroup Excavata. Approximately 150 species of Heterolobosea have been described, from which less than 20 are obligately anaerobic. Most anaerobic species belong to the family Psalteriomonadidae. The aim of this study was to examine the diversity of potentially uncultivable anaerobic heteroloboseids. We obtained multiple new isolates, mainly from freshwater hypoxic sediments, isolated DNA from fresh cultures and determined their SSU rRNA gene sequences using Psalteriomonadidae-specific primers. In total, we have analyzed sequences of 123 new strains, which represented six already described species of Psalteriomonadidae as well as 16 potentially new ones. However, most of the new species died during the early stages of culture. Our results suggest that a great diversity of uncultivable psalteriomonadids exists in the nature. Key words: diversity, SSU rRNA, Heterolobosea, cultivation, Psalteriomonadidae