HPV asociované karcinómy hlavy a krku: morfologická, klinická a molekulovo biologická korelácia

• Main Author: Švajdler, Marián
• Other Authors: Skálová, Alena
• Format: Doctoral Thesis
• Language: Czech
• Published: 2017
• Online Access: http://www.nusl.cz/ntk/nusl-357060

This doctor thesis comprises two studies. (i) A retrospective study was performed on 22 patients who presented with cystic head and neck squamous cell carcinoma (SCC) metastases. The purpose of the study was to find out whether histomorphology, p16 protein expression, HPV and EBVstatus could be useful in predicting the localization of the primary tumor. The primary site was identified in 20 of 22 patients and included the oropharynx in 14 patients (63.6%), the nasopharynx in 3 patients (13.6%), the lungs in 2 cases (9%), and the skin of the auricle in one case (4.5%). No primary was found in two patients (9%). Sixteen of 17 cases (94.1%) originating in Waldayer's ring, and both cases with an unknown primary showed morphology of non-keratinizing SCC or non-keratinizing SCC with maturation. All tumors with oropharyngeal primary and both cases with unknown primary showed diffuse p16 staining and presence of HPV DNA. All three cystic metastases of nasopharyngeal carcinoma (NPCa) were EBV-positive and p16/HPV negative. In contrast, cutaneous and pulmonary metastases showed morphology of a well differentiated keratinizing SCC and poorly differentiated keratinizing SCC, respectively, and were HPV/EBV-negative. We confirmed that cystic SCC lymph node metastases of the head and neck region are strongly...