Vliv monepantelu na expresi biotransformačních enzymů ovce

• Main Author: Štolcová, Tereza
• Other Authors: Skálová, Lenka
• Format: Dissertation
• Language: Czech
• Published: 2015
• Online Access: http://www.nusl.cz/ntk/nusl-348669

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Tereza Štolcová Supervisor:prof. RNDr. Lenka Skálová Ph.D. Title of diploma thesis: Effect of monepantel on expression of biotransformation enzymes in sheep Monepantel (MOP) represents a new anthelmintic drug from the amino-acetonitrile derivatives group with the different mode of action in comparison with commonly used broad-spectrum drugs. It is used for the treatment and control of gastrointestinal nematode infection in sheep and cattle. This study focuses on the effect of MOP on expression of selected cytochromes P450 (CYP) in sheep in vivo and in vitro. The sheep treated with the recommended therapeutic dose of MOP was used for the in vivo experiment. Non-treated animals represented the control group. Changes of mRNA expression of selected CYPs in primary cultures of ovine hepatocytes were observed after 24 h incubation with MOP (10 μM) in the in vitro experiment. The qPCR method (real time quantitative PCR) was used for the quantification of transcription levels of mRNA. Both in vivo as well as in vitro results showed a significant up-regulation in CYP3A24 expression. The induction effect of MOP was even stronger than induction effect of well-known CYP3A inducer rifampicin....