| Summary: | Impact of spermiogramme quality on in vitro fertilization Master's Thesis Author: Bc. Lukáš Gold Field of study: Specialist in laboratory methods Charle's university in Prague Faculty of Pharmacy in Hradec Králové Number of couples who have problems with becoming pregnant increasis every year. Sometimes, change of lifestyle or alleviation of life tempo can help but in other cases couples have to use centre of assisted reproduction service. In some cases is inferility caused by only one factor, in other cases are factors combinated. The aim of this work was evaluation of question, if the spermiogramme quality influences quality of artificial insemination. The secondary aim was to evaluate which technique of oocyte active insemination by injection (Intracytoplasmic sperm injection, ICSI or Intracytoplasmic morphologically - selected sperm injection IMSI) is more successful at fertilization in vitro. Datas and results were taken from 100 couples who underwent therapy using donated oocytes at Zentren Prof. Zech - Pilsen, s.r.o. clinic. Men who gave a fresh ejaculate in day of oocyte collection by ovarian puncture are included in this work. Donated oocytes were micromanipulatively impregnated by spemrs which were taken from ejaculate. Acording to the spermiogramme results men were divided into two...
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