Setup and characterization of an automated method for salt-assisted dispersive liquid-liquid microextraction using a Lab-in-Syringe system

• Main Author: Bešťák, Ondřej
• Other Authors: Horstkotte, Burkhard
• Format: Dissertation
• Language: English
• Published: 2016
• Online Access: http://www.nusl.cz/ntk/nusl-344696

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Ondřej Bešťák Supervisor: Dr. Burkhard Horstkotte, Ph.D., Ms.C. Co-supervisor: PharmDr. Petr Chocholouš, Ph.D. Title of the diploma thesis: Setup and characterization of an automated method for salt-assisted dispersive liquid-liquid microextraction using a lab-in-syringe system Sequential Injection Analysis (SIA) is a technique derived from the Flow Injection Analysis technique. The system generally consists of a computer-controlled syringe pump, a selection valve, and a detector, all connected by inert plastic tubing. It is used to automate laboratory procedures. The "Lab-In-Syringe" technique is a modification of SIA used to carry out parts of the experiment inside the used syringe pump's void. Using a PTFE-coated magnetic-propelled stirring bar inside the syringe allows, for example, to mix homogenously the syringe content or to perform liquid extraction protocols such as dispersive liquid-liquid micro-extraction (DLLME). In this work, the approach to perform salting-out assisted in-syringe DLLME was explored and evaluated for the first time. Starting with a one-phase system, the analyte was extracted from water into n-propanol. For this, a highly-concentrated solution of magnesium...