Analysis of 4-(4-Hydroxyphenyl)butan-2-one in dietary supplements

• Main Author: Bernotová, Ludmila
• Other Authors: Chocholouš, Petr
• Format: Dissertation
• Language: English
• Published: 2016
• Online Access: http://www.nusl.cz/ntk/nusl-344690

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Supervisor: PharmDr. Petr Chocholouš, Ph.D. Consultants: Prof. Marcello Nicoletti Ph.D., PharmDr. Josef Malý, Ph.D. Candidate: Ludmila Bernotová Title of thesis: ANALYSIS OF 4-(4-HYDROXYPHENYL)BUTAN-2-ONE IN DIETARY SUPPLEMENTS Raspberry ketone, (4-(4-hydroxyphenyl)butan-2-one), a key flavouring substance of raspberries, has been used in food supplements to support weight loss for several years. Following diploma thesis determines the present quantities of raspberry ketone in the Raspberry Keto 400 Liquid food supplement available on the Italian market. The presences of synephrine and quercetine in that product are also reported. Firstly, a raspberry ketone molecule was identified in the product by preliminary TLC and confirmed by NMR analysis and mass data. To establish fingerprint profile of the product HPTLC technique was used. To obtain quantitative analysis of raspberry ketone quantities, HPLC-DAD method was performed. The chromatographic separation was performed on a reversed phase column ODS- 2 with gradient elution with acetonitrile-water. In this condition, linearity, inter- and intra-day precision and accuracy were within acceptable values. The developed method showed satisfactory...