Vývoj kapilárně elektroforetické metody pro zjišťování ilegálních příměsí v doplňcích stravy na podporu hubnutí

• Main Author: Vršková, Alena
• Other Authors: Polášek, Miroslav
• Format: Dissertation
• Language: Czech
• Published: 2016
• Online Access: http://www.nusl.cz/ntk/nusl-344118

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Alena Vršková Supervisor: doc. RNDr. Miroslav Polášek, CSc. Consultant: PharmDr. Klára Petrů, Ph.D. Title of Diploma Thesis: Development of capillary electrophoretic method for detection of illegal adulterants in weight loss food supplement Obesity, which is among others associated with the consumption of weight- reduction dietary supplements becomes a global problem. This is related with increased consumption of these products and unfortunately also with the presence of undeclared adulterants in food supplements. Capillary electrophoretic method with contactless conductivity detection for simultaneous determination of 1,3-dimethylamylamine (DMAA), synephrine and hordenine in slimming products was developed and validated and then applied to the assay of these analytes in the Synephrine - REDUCT product. The analysis was performed on silica capillary 58 cm long with internal diameter 50 µm and the BGE was 15 mM α-CD in 2 M acetic acid. The applied voltage was 30 kV and the temperature during analysis was maintained at 25 řC. The samples were analyzed by injection at the short end of the capillary. The content of of synephrine in Synephrine-REDUKT was determined by the method devised. It...