Separační vlastnosti klasických porézních kolon a povrchově porézních kolon v analýze ambroxolu a jeho tří nečistot

• Main Author: Slováček, Jan
• Other Authors: Kozlík, Petr
• Format: Dissertation
• Language: Czech
• Published: 2016
• Online Access: http://www.nusl.cz/ntk/nusl-343431

This work was focused on the comparison of the separation of ambroxol and its three impurities with a column contained fully porous particles (Acquity BEH Shield RP18) and with two core-shell columns (Kinetex EVO C18 a Kinetex C18). Different pH values of buffer (borate buffer with pH = 9.70 and a phosphate buffer with pH = 7.00 and pH = 3.00) and different gradient programs were tested for separation of analytes. Compared to the column with fully porous particles (Acquity BEH Shield RP18), core-shell columns provided shorter analysis time. Lower backpressure and different selectivity depend on a separation system. The best separation conditions were obtained with the column containing fully porous particles (BEH Shield RP18) and gradient according to program Method 2 with the borate buffer. Analysis time was 10 minutes and the last peak had the retention time about 9 min. Autosampler was tempered at 10 ř C and injection volume was 2 μl. The column was tempered at 30 řC. Detection was set at wavelength of 247 nm. Component A contained 10 mM borate buffer with pH = 9.70 and component B was acetonitrile. The best separation was achieved by core-shell columns while phosphate buffer with pH = 7.00 was used. Higher efficiency, smaller peak widths and higher sensitivity were observed. Phosphate buffer...