Charakteristika vertikálně uspořádaných uhlíkových nanotrubic jako platforem pro aplikace v oblasti biosenzorů
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tereza Štipková Supervisor: Assoc. Prof. PharmDr. Lucie Nováková, Ph.D. Supervisor specialist: Assist. Prof. Gulnara Safina, Ph.D. Title of thesis: Working performance of vertically alig...
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| Format: | Dissertation |
| Language: | English |
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2014
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| Online Access: | http://www.nusl.cz/ntk/nusl-339236 |
| Summary: | Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tereza Štipková Supervisor: Assoc. Prof. PharmDr. Lucie Nováková, Ph.D. Supervisor specialist: Assist. Prof. Gulnara Safina, Ph.D. Title of thesis: Working performance of vertically aligned carbon nanotubes as platforms for future biosensing applications Blood glucose monitoring is a crucial part of a modern diabetes therapy. In this work, the multi-walled vertically aligned on-chip grown carbon nanotubes (CNT) were applied in construction of a novel third-generation biosensor. The main focus of this work was to investigate the possibility of future application of the sensor in glucose monitoring. The CNT- based amperometric biosensor was developed by immobilising cellobiose dehydrogenase from Corynascus thermophilus onto the CNT surface by physical adsorption. The highly hydrophobic surface of the nanotubes was functionalized with 0.0001% (w/v) polyvinyl alcohol (PVA) in order to enable the enzyme adsorption. X-ray photoelectron spectroscopy measurements confirmed the successful enzyme immobilization onto PVA modified CNT surface. The surface topography and elemental composition of the nanomaterial were examined by scanning electron microscopy and energy-dispersive X-ray spectroscopy... |
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