| Summary: | C-reactive protein, cardiovascular diseases and endothelial dysfunction Diploma thesis Author: Kristýna Bartoňková Study program: Pharmacy Charles University in Prague, Faculty of Pharmacy in Hradec Králové Background: Healthy endothelium significantly affects the vascular homeostasis which is damaged during the endothelial dysfunction. This dysfunction is linked to number of cardiovascular diseases. Endothelial dysfunction, together with the chronic inflammation, represents also basis of the metabolic syndrome. The aim of the thesis was to detect selected markers of endothelial dysfunction in recently published transgenic rat model of metabolic syndrome. Methods: In this thesis, following factors were evaluated: endothelial nitric oxide synthase, phosphorylated endothelial nitric oxide synthase, superoxide dismutase 3, cyclooxygenase 2, nuclear factor κB and forkhead box P3. Evaluation of their expression in the aorta homogenates was performed by method Western blot. The control group was composed of spontaneously hypertensive male rats, the evaluated group was made from transgenic spontaneously hypertensive male rats expressing human C-reactive protein. Then the detection of proteins was performed by chemiluminiscent method on X-ray films. All the data were evaluated by densitometry method....
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