Sledování exprese vybraných markerů funkce endotelu u myší s vysokými hladinami solubilního endoglinu
Bc. Sabina Škodová Study of the expression of some markers of endothelial function in mice with high levels of soluble endoglin. Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Medical laboratory technician Background: The aim of this diploma thesis was to describe...
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| Format: | Dissertation |
| Language: | Czech |
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2015
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| Online Access: | http://www.nusl.cz/ntk/nusl-335142 |
| Summary: | Bc. Sabina Škodová Study of the expression of some markers of endothelial function in mice with high levels of soluble endoglin. Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Medical laboratory technician Background: The aim of this diploma thesis was to describe changes of expression eNOS and ICAM-1 in the wall of aorta of mice with low and high levels of soluble form of endoglin (Sol-Eng+). The attempt was to compare expression of both markers from the position of intensity and also from the position of area of staining. For this purpose we used immunohistochemical methods with detection in fluorescent microscope. Methods: For this study were used transgenic mice with high levels of human soluble endoglin (Sol-Eng+). Female mice were fed from their six months of age with high fat diet, which contained 1.25% of cholesterol and 40% of fat. The mice were fed with this diet for another three months. The same old female mice from the same brood with low level of soluble endoglin in plasma were used as a control group. The expression of eNOS and ICAM- 1 was defined on sections of aorta of the mice with fluorescent immunohistochemistry. Results: Immunohistochemical analysis proved weak expression of eNOS in both groups of mice, and only on vascular endothelium. The... |
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