Vplyv vybraných izoflavonoidov na aktivitu enzýmov podieľajúcich sa na biotransformácii cytostatík

• Main Author: Verešová, Erika
• Other Authors: Svobodová, Hana
• Format: Dissertation
• Language: Slovak
• Published: 2015
• Online Access: http://www.nusl.cz/ntk/nusl-335136

1 ABSTRACT Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Erika Verešová Supervisor: PharmDr. Hana Bártíková, Ph.D. Title of diploma thesis: The impact of selected isoflavonoids on the activity of enzymes participating in cytostatic drugs biotransformation More and more people are suffering from cancer. Scientists are striving to turn to nature and find the help there. One of the possible answers to their questions would be usage of natural substances in either prevention or even treatment of cancer. Isoflavonoids, polyphenolic compounds occurring e.g. in soybeans, are one of them. Many scientists have already tested their influence on the human body. This thesis focuses on the possible effect of isoflavonoids on the activity of enzymes participating in biotransformation of cytostatic drugs. Experiments were performed on human breast cancer cell lines MCF-7 and MDA- MB-231 exposed to isoflavonoids for 24, 48 and 72 hours. Cell line MCF-7 showed greater changes in the enzymatic activity than MDA-MB-231. Of the four enzymes tested (AKR1A1, AKR1C, CBR and GST), two enzymes showed no change in activity after exposure to isoflavonoids - enzymes CBR and AKR1C. Only one experiment brought the desired result for all isoflavones, genistein, daidzein...