Stanovení exprese mRNA enzymu DHRS3 v lidských tkáních pomocí real-time SYBR green qPCR

• Main Author: Rusnáková, Pavlína
• Other Authors: Zemanová, Lucie
• Format: Dissertation
• Language: Czech
• Published: 2015
• Online Access: http://www.nusl.cz/ntk/nusl-332588

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Pavlína Rusnáková Supervisor: RNDr. Lucie Zemanová, PhD. Title of diploma thesis: Determination of mRNA of DHRS3 enzyme in human tissues by real-time SYBR green qPCR Dehydrogenase/reductase (SDR family) member 3 (DHRS3, also known as SDR16C1, retSDR1) is NADPH dependent carbonyl reductase, member of the short-chain dehydrogenases/reductases superfamily. DHRS3 was characterized for the first time by Haeseleer et al. as an enzyme that reduces all-trans-retinal to all-trans-retinol in the visual cycle that is localised in the outer segment of the cone photoreceptors in bovine retina. However, expression of DHRS3 was shown in some other tissues. It was proven that DHRS3 plays an important role in embryogenesis and tumour development. It was also established that DHRS3 participates in the reduction of some endogenous compounds (androstendione, estrone, DL-glyceraldehyde) and xenobiotics (acetohexamide, 4-nitroacetophenone, benzil) in vitro. DHRS3 seems to be an important enzyme with participation in significant metabolic pathways. Nevertheless, knowledge of some DHRS3 properties, including expression in human tissues, is quite poor. The aim of this study was to determine expression of DHRS3...