| Summary: | CHARLES UNIVERSITY IN PRAGUE Third Faculty of Medicine Prague 2014 PhD Thesis Virulence profiles of clinical isolates of enterohemorrhagic Escherichia coli and their relationship to disease severity ing. Monika Marejková Summary Enterohemorrhagic Eschericha coli (EHEC) cause diarrhea and diarrhea-associated (D+) hemolytic-uremic syndrome (HUS) worldwide. D+ HUS develops as Shiga toxin (Stx)- mediated systemic complication of diarrhea mostly in children under 5 years and is the most common cause of acute renal failure in childhood. The aim of this study was to determine the role of EHEC in etiology of HUS and diarrhea in the Czech Republic, to develop rapid and reliable methods for EHEC detection and molecular subtyping, to analyze virulence gene profiles of the EHEC isolates and their correlation with the clinical outcome of the infection (i.e. uncomplicated diarrhea vs. HUS) and to gain insight into the epidemiology of EHEC infections. During 1998-2012 EHEC were isolated from stool samples of 70 % of pediatric patients with HUS. The isolates belonged to serotypes [fliC types] O157:H7/NM[fliCH7] (50 % were sorbitol-fermenting strains), O26:H11/NM[fliCH11], O55:NM[fliCH7], O111:NM[fliCH8], O145:H28[fliCH28], O172:NM [fliCH25], and Orough:NM[fliCH25]. EHEC O26 accounted for 41 % of all isolates. More than one...
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