| Summary: | Since 1980s when the functions of nitric oxide were discovered, it is known that this compound plays an important role in many physiological processes like cell-cell communication, vascular relaxation, and also in development of pathophysiological disorders like neurodegeneration and vascular dysfunctions. The present work focuses on in-situ amperometric detection of nitric oxide releasing from living pheochromocytoma (PC12) single cells using the developed microelectrodes modified with electrodeposited platinum nanoparticles. Developed platinization method was successfully applied also for fabrication of 2-, 3- and 7-barrels microelectrode arrays. Time of platinum deposition and scan rate were found and optimized in order to achieve both sufficient but not excessive coverage of the electrode with Pt nanoparticles, and acceptable sensitivity towards the measured target. It was found, that scanning of potential between -0.2-1.0 V for 10 cycles with scan rate of 70 mVs-1 leads to formation of reproducible uniform layer of Pt nanoparticles with size of ca. 70 nm and increased sensitivity towards the oxygen and nitrogen reactive species. It was found, that the Pt modified carbon fiber microelectrode makes it possible to detect hydrogen peroxide with concentration as low as 62nM. Amperometric...
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