| Summary: | Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Candidate: Kateřina Burdová Supervisor: PharmDr. Petr Kastner, Ph.D. Title of diploma thesis: Study of UHPLC separation depending on temperature This thesis analyzes the UHPLC separation and its dependence on temperature. The aim of this project was to map the temperature dependence of the individual chromatographic parameters: retention time, symmetry factor, separation factor and resolution. Seven different commonly used methods were applied to analyze different chemical substances. These include acids, bases and neutral substances. Temperature range at Kinetex C18 column was 25 ř C to 130 ř, 150 x 2.10 mm. The manufacturer guarantees stability of this column at up to 60 řC. As a part of the analysis, the column was exposed to a temperature of 130 ř C for a short period of time. No degradation of the column was observed. The research demonstrated that in all cases, the retention time along with the separation factor decrease as the temperature increases. Similarly, the resolution worsened as the temperature increased and it was only calculated if the individual substances were sufficiently separated throughout the whole temperature range tested. The time of analyzes was...
|
|---|
