| Summary: | Jana Šindelářová Alteration of transport proteins expression during obstructive cholestasis in rats I Diploma thesis Charles University in Prague, Fakulty of Pharmacy in Hradec Králové Pharmacy Background: The purpose of the diploma thesis was the confirmation of liver cholestatic damage induced by bile duct obstruction lastnig for 28 days by biochemical analysis and analysis of mRNA and protein expression of liver uptake transporters in rats (Ntcp, Oatp1a1, Oatp1a4, Oatp1b2, Oat2). Methods: Wistar rats (n = 6, in each group; 280 - 320g) were dividend in two groups: control group of sham-operated animals (Sham) and group with bile duct obstruction lastnig for 28 days (BDO). Biochemical analysis of serum was performed by Cobas Integra ® 800. Changes of mRNA and protein expression of transport proteins were evaluated by qRT-PCR and Western blot. Results: In BDO group, level of bile acids increased to 454%, level of total bilirubin to 4111% and level of conjugated bilirubin to 7313% as compared to control group. In comparison to control group, activity of ALP was elevated to 238%, activity of GMT to 2826%, activity of ALT to 1200% and activity of AST to 1378%. As compared to Sham group BDO group showed decreased mRNA levels of Ntcp to 51% and of Oatp1a4 to 38%. No signifiant changes were found in...
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