Vývoj UHPLC-MS/MS metody pro stanovení skupiny statinů a jejich metabolitů

• Main Author: Svoboda, Pavel
• Other Authors: Nováková, Lucie
• Format: Dissertation
• Language: Czech
• Published: 2013
• Online Access: http://www.nusl.cz/ntk/nusl-325126

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Pavel Svoboda Supervisor: Doc. PharmDr. Lucie Nováková, Ph.D. Title of Graduation Thesis: Development of UHPLC-MS/MS method for the determination of statins and their metabolites This graduation thesis deals with development of the method for the determination of statins and their metabolites by ultra-high performance liquid chromatography coupled to tandem mass spectrometry. First, the chromatographic conditions were optimized. Next, the parameters of mass spectrometer were optimized. And finally, repeatability, linearity and sensitivity of the method were assessed. Chromatographic column BEH C18 (50 x 2.1 mm, 1.7 µm) was used for the analysis. The choice of column was performed using UV spectrophotometric detector. The mobile phase of the gradient elution consisted of 0.1 mM ammonium acetate (A) and acetonitrile (B). The initial ratio of mobile phase A:B was set at 70:30. The optimization of the mass spectrometry parameters started with the selection of precursor ions using direct infusion. Thereafter, the ion source parameters of the mass spectrometer were optimized. The ion transitions were optimized after the selection of product ions. The optimization of ion transitions was...