Studium přípravy fluorescenčně značeného bilirubinu

• Main Author: Felklová, Veronika
• Other Authors: Smrček, Stanislav
• Format: Dissertation
• Language: Czech
• Published: 2013
• Online Access: http://www.nusl.cz/ntk/nusl-324191

Heme is a prosthetic group containing iron atom at the center of the tetrapyrrole cycle, which is the part of hemoglobin. Heme is metabolized by heme oxygenase to linear tetrapyrrolic pigment - biliverdine. It is immediately transformed to bilirubin by biliverdin reductase. This pigment is excreted daily into the systematic circulation. Normal levels of unconjugated bilirubin in plasma are 5 - 17 μmol/l for adult. At extremely elevated levels (above 340 μmol / l) causes cytotoxic due to its lipophility, especially in the neonatal period can lead to the development of bilirubin encephalopathy. The pathogenesis of this complication is not clear, there is no precise information of the transport of unconjugated bilirubin across the blood-brain barrier as well as target structures in the central nervous system. Distribution of unconjugated bilirubin in newborns is still not entirely clear. The aim of this work is to discover new methods which would allow more detailed investigation of distribution of bilirubin in living systems. A suitable method appears to be fluorescent labelling of tetrapyrrole pigment in position C10 which reaches into the spatial arrangement of the molecule, and does not affect its physicochemical properties and thus its biological activity. The fluorescence-labeled molecules can...