| Summary: | Pseudomonas aeruginosa is an opportunistic human pathogen that is commonly found in nature. Pathogenesis of P. aeruginosa is related especially to cystic fibrosis, where the lung infection with this microorganism is a leading cause of death of patients. Major complication is chronic colonization and infection of the patients airway by specific microorganisms - P. aeruginosa, B. cepacia complex. The colonization of respiratory tract is mediated by the series of adhesive structures such as lectin PA-IIL. Currently, the most widely used therapy is an antibiotic treatment. Due to the increasing resistance to antibiotics another methods for treatment are being searched. One possibility is a passive immunization of patients with chicken antibodies. For this purpose, we prepared antibodies against one of the adhesive structures of P. aeruginosa - recombinant lectin PA- IIL. The ability of antibodies to prevent the adhesion of bacteria to the human lung cells was studied in an in vitro model system based on human lung cells and P. aeruginosa. We used stable cell lines A549, NuLi-1, CuFi-1. The process of adhesion tests was optimized. To evaluate the adhesion tests we searched for an appropriate objective evaluation method based on the quantification of fluorescence of the components of the model system. We...
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