Chemiluminiscenční stanovení vitamínu B12 pomocí průtokových technik analýzy

This thesis reports determination of vitamin B12 by flow techniques of analysis. This method is based on luminol-peroxide chemiluminiscence assay for the detection of cobalt (II) ions in vitamin B12 molecules. The method includes releasing of Co2+ from the vitamin B12 using different types of its de...

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Main Author: Svobodová, Kateřina
Other Authors: Hraníček, Jakub
Format: Dissertation
Language:Czech
Published: 2013
Online Access:http://www.nusl.cz/ntk/nusl-322178
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spelling ndltd-nusl.cz-oai-invenio.nusl.cz-3221782017-06-28T04:17:41Z Chemiluminiscenční stanovení vitamínu B12 pomocí průtokových technik analýzy Determination of vitamin B12 by flow analysis with chemiluminiscent detection Svobodová, Kateřina Hraníček, Jakub Rychlovský, Petr This thesis reports determination of vitamin B12 by flow techniques of analysis. This method is based on luminol-peroxide chemiluminiscence assay for the detection of cobalt (II) ions in vitamin B12 molecules. The method includes releasing of Co2+ from the vitamin B12 using different types of its decomposition; these are mentioned in this thesis. The efficiency of the decomposition of real samples of vitamin B12 in this study reached 74.43 %. The high efficiency was achieved with 1.5 mmol.l-1 K2S2O8 and irradiation time 1 minute. In the FIA mode, the linear range obtained was between 1.0 to 500 mg.l-1 with a relative standard deviation (RSD) 2.59% (n = 10), limit of detection (LOD) 55 µg.l-1 . The minimum sample volume required and the time of analysis were 60 µl and 140.2 s. In the SIA mode, the linear range obtained was between 0.01 to 50 mg.l-1 with an RSD = 4.5% (n = 10), LOD = 25 µg.l-1 . The minimum sample volume required and the time of analysis were 250 µl and 80.1 s. Interference study showed that from wide range of interfering ions were selected only Ni2+ , Zn2+ and NO2 - , that from concentration level 50 mg.l-1 they significantly affect the determination of the Co2+ in solution. The intensity of chemiluminiscence most suppress ion Zn2+ . The solution with the concentration ratio of Co2+... 2013 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-322178 cze info:eu-repo/semantics/restrictedAccess
collection NDLTD
language Czech
format Dissertation
sources NDLTD
description This thesis reports determination of vitamin B12 by flow techniques of analysis. This method is based on luminol-peroxide chemiluminiscence assay for the detection of cobalt (II) ions in vitamin B12 molecules. The method includes releasing of Co2+ from the vitamin B12 using different types of its decomposition; these are mentioned in this thesis. The efficiency of the decomposition of real samples of vitamin B12 in this study reached 74.43 %. The high efficiency was achieved with 1.5 mmol.l-1 K2S2O8 and irradiation time 1 minute. In the FIA mode, the linear range obtained was between 1.0 to 500 mg.l-1 with a relative standard deviation (RSD) 2.59% (n = 10), limit of detection (LOD) 55 µg.l-1 . The minimum sample volume required and the time of analysis were 60 µl and 140.2 s. In the SIA mode, the linear range obtained was between 0.01 to 50 mg.l-1 with an RSD = 4.5% (n = 10), LOD = 25 µg.l-1 . The minimum sample volume required and the time of analysis were 250 µl and 80.1 s. Interference study showed that from wide range of interfering ions were selected only Ni2+ , Zn2+ and NO2 - , that from concentration level 50 mg.l-1 they significantly affect the determination of the Co2+ in solution. The intensity of chemiluminiscence most suppress ion Zn2+ . The solution with the concentration ratio of Co2+...
author2 Hraníček, Jakub
author_facet Hraníček, Jakub
Svobodová, Kateřina
author Svobodová, Kateřina
spellingShingle Svobodová, Kateřina
Chemiluminiscenční stanovení vitamínu B12 pomocí průtokových technik analýzy
author_sort Svobodová, Kateřina
title Chemiluminiscenční stanovení vitamínu B12 pomocí průtokových technik analýzy
title_short Chemiluminiscenční stanovení vitamínu B12 pomocí průtokových technik analýzy
title_full Chemiluminiscenční stanovení vitamínu B12 pomocí průtokových technik analýzy
title_fullStr Chemiluminiscenční stanovení vitamínu B12 pomocí průtokových technik analýzy
title_full_unstemmed Chemiluminiscenční stanovení vitamínu B12 pomocí průtokových technik analýzy
title_sort chemiluminiscenční stanovení vitamínu b12 pomocí průtokových technik analýzy
publishDate 2013
url http://www.nusl.cz/ntk/nusl-322178
work_keys_str_mv AT svobodovakaterina chemiluminiscencnistanovenivitaminub12pomociprutokovychtechnikanalyzy
AT svobodovakaterina determinationofvitaminb12byflowanalysiswithchemiluminiscentdetection
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