Studium ledvinné akumulace antivirotik s využitím buněčného modelu

• Main Author: Navrátilová, Lucie
• Other Authors: Trejtnar, František
• Format: Dissertation
• Language: Czech
• Published: 2013
• Online Access: http://www.nusl.cz/ntk/nusl-322062

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Lucie Navrátilová Supervisor: Doc. PharmDr. František Trejtnar, CSc. Title of diploma thesis: Study of renal accumulation of antivirals using cellular model Administration of antiviral drugs may be in some case associated with severe nephrotoxicity. This adverse effect can be based on active renal transport and accumulation of the drugs in the renal cells. Several experimental in vitro methods are available to study the mechanisms responsible for drug uptake in the kidney including standard renal cell lines or renal slices. The aim of this study was to evaluate usefulness of isolated rat renal cells to determine which renal transporters may be involved in transport of selected antivirals into the renal cells. The freshly isolated rat renal cells were prepared by two-phase collagenase perfusion method. To evaluate contribution of active and passive transport mechanisms to the renal uptake, accumulation at normal and low incubation temperature was evaluated. To confirm previous information on mechanisms of the renal transport, we also determined which groups of transporters contributed to the renal accumulation of the studied antivirals. For this purpose, we use specific inhibitors of...