Hodnocení vybrané účinné látky v přípravku VI.

• Main Author: Zavadilová, Hana
• Other Authors: Kastner, Petr
• Format: Dissertation
• Language: Czech
• Published: 2011
• Online Access: http://www.nusl.cz/ntk/nusl-313335

1 ABSTRACT Determination of Selected Active Substance in the Preparation VI. Thesis Hana Zavadilová Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control Suitable UPLC methods for the determination of cinchocainum-hydrochloridum and for the determination purity of dexamethasonum were searched. The chromatographic column Kinetex™ C18 (150 x 2,1 mm, 1,7 µm) was chosen. A suitable mobile phase for the determination of cinchocainum-hydrochloridum was a mixture of acetonitrile:solution of triethylamine in concentration 30 mmol/l (1,05 ml triethylamine in 250 ml of water), 75:25. pH of the mobile phase was adjusted to 7,0 by orthophosphoric acid. Flow rate was 0,2 ml/min. UV detector scanned at 325 nm. The column temperature was maintained at 30 řC. Linearity, repeatability and accuracy were tested under these conditions. Method for determination purity of dexamethasonum was running under conditions of gradient elution. Composition of mobile phase: Mobile phase A was acetonitrile:water, 30:70. Mobile phase B mixture of acetonitrile:solution of triethylamine in concentration 30 mmol/l (1,05 ml triethylamine in 250 ml of water), 75:25. pH of the mobile phase was adjusted to 7,0 by orthophosphoric acid. Flow rate 0,2 ml/min. Detection UV at 238...