Elektroforetické stanovení 8-hydroxy-2-deoxyguanosinu jako markeru oxidativního stresu

• Main Author: Bydžovská, Lucie
• Other Authors: Polášek, Miroslav
• Format: Dissertation
• Language: Czech
• Published: 2012
• Online Access: http://www.nusl.cz/ntk/nusl-308210

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Anylytical Chemistry Candidate: Lucie Bydžovská Supervisor: . RNDr. Miroslav Polášek, CSc. Title of diploma thesis: Electrophoretic determination of 8-hydroxy-2'-deoxyguanosine as a marker of oxidative damage Abstract 8-Hydroxy-2'-deoxyguanosine (8-OHdG) is probably the most important and well-documented product of oxidative DNA damage in biological systems. The study of oxidative DNA destruction is valuable in biomedical research and in diagnostics concerning cancer and ageing. Capillary electrophoresis (CE) has been tested as a highly efficient separation method for the analysis of 8-OHdG as an alternative to conventionally used HPLC. The aim of this work was to develop CE/UV method for the determination of 8-OHdG in urine samples. Optimal CE separation of 8-OHdG from other urine components was achieved with background electrolyte based on 150mM borate buffer of pH* 9.2 (adjusted with NaOH) containing 10% (v/v) of acetonitrile; 2'-deoxyguanosine was selected as optimal internal standard (IS). The detection wavelength was 282 nm. The samples analysed were standard aqueous solutions of 8-OHdG + IS and human urine spiked with 8-OHdG + IS. For increasing the sensitivity of the CE special separation capillary equipped with a...