Transport a biotransformace anthelmintik u citlivých a rezistentních kmenů vlasovky slézové (Haemonchus contortus)

• Main Author: Prchal, Lukáš
• Other Authors: Szotáková, Barbora
• Format: Dissertation
• Language: Czech
• Published: 2012
• Online Access: http://www.nusl.cz/ntk/nusl-307830

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Lukáš Prchal Supervisor: Doc. Ing. Barbora Szotáková, Ph.D. Title of diploma thesis: Transport and biotransformation of anthelmintics in susceptible and resistant strains of Barber's pole worm (Haemonchus Contortus) Haemonchosis is a worldwide problem, which endanger breeding of small ruminants. This disease is caused by nematode Barber's pole worm (Haemonchus contortus), which parasites inside stomachs of host animals, causing anaemia and in many cases death. Pharmacotherapy is the most used way to prevent and/or threat animals. The use of benzimidazole anthemintics is very common, but it's failing more and more often because of resistance of parasites to pharmacotherapy. The most common reason of resistance is mutation in the gene for β-tubuline, which is target for benzimidazoles. This research focuses at the other possible mechanism of resistance, which is resistance based on enzymes and metabolism. One of the aims of this thesis was to compare the metabolism of the anthelmintic drug flubendazole in three different strains of H. contortus: the ISE strain (susceptible to common anthelmintics), the BR strain (resistant to benzimidazole anthelmintics) and the WR strain (resistant to all...