Neurotropní a antioxidační aktivita vybraných druhů jednoděložných alkaloidních rostlin. II.

• Main Author: Benešová, Nina
• Other Authors: Cahlíková, Lucie
• Format: Dissertation
• Language: Czech
• Published: 2012
• Online Access: http://www.nusl.cz/ntk/nusl-307083

Benešová N.: Neurotropic and antioxidative activity in vitro of monocotyledonous alkaloidal plants II. Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Departement of Pharmaceutical Botany and Ecology, Hradec Králové 2012, 72 p. The aim of this diploma thesis was to analyse alkaloidal extracts of six selected plants from Amaryllidaceae family (Nerine filifolia, Nerine undulata, Nerine filamentosa, Scadoxus multiflorus, Sprekelia formosissima, Sternbergia lutea) and test their biological activity to human cholinesterases (HuAChE, HuBuChE) using Ellman's method and antioxidative activity measured by DPPH test. Thanks to GC/MS analysis was identified a huge range of alkaloids of Amaryllidaceae family that belongs to various structural types. Some of them have not been determined yet due to unavailability of their spectras in commercial libraries and literature. The best inhibitory activity against human blood acetylcholinesterase was described in alkaloidal extracts of Nerine undulata (IC50 14,30 ± 1,20 µg/ml) and Nerine filifolia (IC50 18,54 ± 0,79 µg/ml) and against human plasma butyrylcholinesterase in extracts of Sternbergia lutea (IC50 3,07 ± 0,05 µM) and Nerine filamentosa (IC50 13,00 ± 0,71 µg/ml). Antioxidative activity of alkaloidal extracts from the bulbs...