Průnik nanočástic sublingvální membránou 2

• Main Author: Dvořáková, Pavlína
• Other Authors: Berka, Pavel
• Format: Dissertation
• Language: Czech
• Published: 2012
• Online Access: http://www.nusl.cz/ntk/nusl-304095

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of pharmaceutical technology Author: Dvořáková Pavlína Supervisor: Mgr. Pavel Berka Title of Diploma Thesis: Permeation of nanoparticles across sublingual membrane 2 In theoretical part of this work, the anatomy and physiology of oral cavity and tongue, sublingual application of active substances, nanoparticles are reviewed. Instruments used for measurements are also described. In the following experimental part, the permeation of Chromeon 470 marked nanoparticles through porcine sublingual membrane into buffer pH 7,4 (acceptor phase) in vitro was evaluated. Sublingual membrane with surface 1 cm2 was saturated by buffer pH 6,6. During the first experiment, frozen sublingual membrane (19. 1. 2011) was used. It was thawed out just before the experiment (7. 9. 2011). In the case of the second experiment, fresh sublingual membrane, which was shortly immersed in the physiological solution, was used (14. 9. 2011). Nanoparticles permeated better through a long-time frozen porcine membranes than through freshly cut up membranes. The nanoparticles permeated better from diluted samples than from non-diluted samples. Keywords: Anatomy and physiology of oral cavity and tongue, nanoparticles, Chromeon 470, sublingual application,...