Vliv extracelulární matrix na expresi genů jaterních myofibroblastů

• Main Author: Jiroutová, Alena
• Other Authors: Kanta, Jiří
• Format: Doctoral Thesis
• Language: Czech
• Published: 2011
• Online Access: http://www.nusl.cz/ntk/nusl-299472

Influence of extracellular matrix environment on gene expression in liver myofibroblast (summary) Hepatic stellate cells (HSC) and liver myofibroblasts (MF) are two cell populations most likely responsible for the synthesis of majority of connective tissue components in fibrotic liver. They differ in their origin and location in the liver, and in the spectrum of genes they express. HSC are located in Disse spaces of normal rat liver around the sinusoids, in fibrotic liver they become activated, proliferate and they undergo transdifferentiation into myofibroblast-like cells. Myofibroblasts are heterogenous cell population that consists at least of portal pMF, septal sMF and interface iMF. pMF, which are adjacent to bile duct epithelia, may be a mediator of billiary type fibrosis. sMF are located within and along the collagenous septum in cirrhotic liver. Little is known about the expression of genes involved in connective tissue metabolism in MF cultured in fibrin or collagen gels that more closely resemble natural cell environment. Fibrin is deposited in liver at sites of injury and collagen type I forms a substantial part of fibrotic septa. In our study oligo cDNA array analysis was used to determine gene expression in quiescent HSC, activated HSC and MF isolated from both normal and CCl4-cirrhotic liver....