Vývoj a validace HPLC metody pro stanovení ketoprofenu v čípcích
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Lukáš Zahálka Supervisor: PharmDr. Ludmila Matysová, Ph.D. Title of Diploma Thesis: Development and validation of HPLC method for the determination of ketoprofen in suppositories The pur...
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| Format: | Dissertation |
| Language: | Czech |
| Published: |
2011
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| Online Access: | http://www.nusl.cz/ntk/nusl-297976 |
| Summary: | Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Lukáš Zahálka Supervisor: PharmDr. Ludmila Matysová, Ph.D. Title of Diploma Thesis: Development and validation of HPLC method for the determination of ketoprofen in suppositories The purposes of diploma thesis were development and validation of HPLC method for the determination of ketoprofen in Ketonal® 100 mg suppositories. Ketoprofen belongs to NSAID and it is used for symptomatic therapy of inflammatory, degenerative and metabolic rheumatic diseases and for palliative therapy of some urgent or chronic painful syndromes. During development of determination of ketoprofen in suppositories were used two methods, which were developed at the Faculty of Pharmacy in Hradec Králové. Method for determination of diclofenac in suppositories was default for sample preparation and method of HPLC determination of ketoprofen, methylparaben and propylparaben in gel was basic for conditions of HPLC analysis. Development of method consisted in modification of origin sample preparation method; chromatographic conditions have not been changed. During the experimental work was found to determine what influence has the various steps of sample preparation and extraction temperature. The time of extraction... |
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