| Summary: | Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Adriana Dudová Supervisor: PharmDr. Marie Kašparová, PhD. Title of Doctoral Thesis: Explant Culture of Trifolium pratense L. The objective of this thesis was to observe the effect of the abiotic elicitors of aluminium chloride and chromium chloride on the production of flavonoids, as well as the effect of the aluminium chloride elicitor on the production of isoflavonoids in the Trifolium pratense L. exulant culture (variety DO-9). The cultures were cultivated in the Gamborg nutrient media with the addition of 2 mg.l¯¹ of 2,4-dichlorophenoxyacetic acid and 2 mg.l¯¹ of 6-benzylaminopurine, at the temperature of 25 řC, 16-hr light/8-hr dark period. The quantity of flavonoids was determined spectrophotometrically according to the Czech Pharmacopoeia 2009. The quantity of isoflavonoids was determined using HPLC. The results of the elicitation of the Trifolium pratense L. suspension culture with aluminium chloride and chromium chloride show that the maximum increase in the flavonoid production compared to the control culture was evoked by the application of a 100 µmol concentration over a 168-hr period in both cases. During elicitatiton of the Trifolium pratense L. exulant culture (variety DO-9)...
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