Sledování reaktivity kožního alergenu limonenu-1-hydroperoxidu s aminokyselinami a identifikace jeho aduktů pomocí LC/ESI-MS/MS

• Main Author: Škrášková, Karolina
• Other Authors: Šatínský, Dalibor
• Format: Dissertation
• Language: English
• Published: 2011
• Online Access: http://www.nusl.cz/ntk/nusl-296398

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Karolina Škrášková Consultant: Doc. RNDr. Dalibor Šatínský, PhD. Diploma Thesis Title: Investigation of the reactivity of the skin allergen limonene-1-hydroperoxide with amino acids and identification of adducts with LC/ESI- MS/MS. Contact allergy and its clinical manifestation allergic contact dermatitis represent a serious problem in western countries. Thousands of chemicals have been proven to cause skin sensitization followed usually by an inflammation of the skin. Limonene is a monoterpene used as a fragrance compound in household products and cosmetics. It is itself a non-allergenic compound. Nevertheless after an air exposure it creates oxidized products, which are potent allergens. The hydroperoxides fraction of the oxidation mixture has been shown to be strongly skin sensitizing. Two of the major compounds in the fraction are limonene-1-hydroperoxide and limonene-2-hydroperoxide. During decades of research devoted to the contact allergy it was revealed, that the chemicals causing allergic contact dermatitis react with skin proteins, thus starting up a complicated cascade of immunological processes leading to the final skin irritation. The reaction between the chemical and the...