Elektroforetické stanovení vybraných myorelaxancií s využitím bezkontaktní vodivostní detekce
A novel capillary electrophoretic method for the separation of pancuronium (PM) and vecuronium (VM) ions utilizing capacitively coupled contactless conductivity detection (C4 D) was devised and validated. The separation was carried out in bare fused-silica capillaries (50 µm i.d., 75/45 cm) at 25 řC...
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| Format: | Dissertation |
| Language: | Czech |
| Published: |
2011
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| Online Access: | http://www.nusl.cz/ntk/nusl-295995 |
| Summary: | A novel capillary electrophoretic method for the separation of pancuronium (PM) and vecuronium (VM) ions utilizing capacitively coupled contactless conductivity detection (C4 D) was devised and validated. The separation was carried out in bare fused-silica capillaries (50 µm i.d., 75/45 cm) at 25 řC. Optimal background electrolyte was 50 mM borate buffer of pH 9.5 containing 12.5 mg/ml of hydoxypropyl-γ-cyclodextrine; (phenyltrimethylammonium iodide was used as internal standard). The samples were injected hydrodynamically at 1000 mbar for 3 s. The separation was performed at +30 kV. Under such conditions the PM and VM were base-line resolved and the separation took < 4 min. Calibration curves were linear for both PM bromide and VM bromide in the range 25-250 μg/ml with r > 0.9968. The limits of detection were 7 and 6 μg/ml for PM bromide and VM bromide, respectively. The accuracy tested by recovery experiment at three concentration levels of added PM bromide and VM bromide was satisfactory (95.7-102.7 %, n=3, with RSD < 2.61 %). The method was successfully applied to the assay of PM bromide and VM bromide in commercial injection solutions. |
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