Kultury léčivých rostlin in vitro - III

• Main Author: Danielová, Barbora
• Other Authors: Tůmová, Lenka
• Format: Dissertation
• Language: Czech
• Published: 2008
• Online Access: http://www.nusl.cz/ntk/nusl-292621

- 4 - ABSTRAKT Barbora Danielová In vitro-cultures of medicinal plants-III. Elicitation is one of the methods that can be used for increasing secondary metabolites production in in vitro cultures. The present study investigates the effect of methylviologen as the abiotic elicitor on the flavonoids production in the callus and suspension cultures of Ononis arvensis L. after 6, 12, 24, 48, 72 and 168-hours methylviologen administration. The cultures were cultivated on Murashige-Skoog medium with addition of 10 mg/l α-naphtylacetic acid (α-NAA). The flavonoids content was determined using spectrophotometry methods. The maximal content of flavonoids was detected in callus culture after 168-hours methylviologen elicitation in concentration c1 (2,1929 . 10-3 mol/l), the production was increased about 444%. The maximal increase of flavonoids production in suspension culture was after 12-hours methylviologen elicitation in concentration c3 (2,1929 . 10-5 mol/l), the production was increased about 42%. In the opposite, the decreased flavonoids production was detected in callus culture after methylviologem treatment in the concentration c1 (2,1929 . 10-3 mol/l) after 6 and 12 hours about 100% and in suspension culture in the concentration c2 (2,1929 . 10-4 mol/l) after 12 hours about 88%.