Podíl subtypů P2 receptorů na modulaci glutamatergního přenosu v mozkové kůře
Glutamate is the major excitatory neurotransmitter in the central nervous system, and there is emerging evidence that its release is subject to presynaptic regulation by P2 receptors. Activation of P2X receptors elicited glutamate release from terminal of dorsal horn neurons of spinal cord and hippo...
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| Format: | Dissertation |
| Language: | English |
| Published: |
2008
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| Online Access: | http://www.nusl.cz/ntk/nusl-292618 |
| Summary: | Glutamate is the major excitatory neurotransmitter in the central nervous system, and there is emerging evidence that its release is subject to presynaptic regulation by P2 receptors. Activation of P2X receptors elicited glutamate release from terminal of dorsal horn neurons of spinal cord and hippocampus whereas activation of P2Y receptors has been shown to inhibit glutamate release in the hippocampus. Glial cells express several subtypes of P2Y receptors and there is growing evidence that these cells are active elements at synapses, they release ATP upon activation of glutamate receptors and participate in the regulation of glutamatergic transmission. The aim of the present study was to investigate the role of P2Y receptors in the regulation of glutamatergic transmission in rat brain cortex; whether uptake of glutamate by glial cells is influenced by P2Y receptor-activation and to identify the subtypes of P2Y receptors involved. Primary cultures of cortical astrocytes obtained from brain hemispheres of newborn rats were used as a model to study the influence of P2Y receptors on the uptake of glutamate. We clarified the role of several P2Y agonists and we identified the subtypes of P2Y receptor involved in the regulation of synaptic concentration of glutamate which may provide the knowledge for... |
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