Sledování účinku atorvastinu na proces aterogeneze u apoE-/-/LDLR-/ - double knock-out myšího modelu aterosklerózy III.

• Main Author: Slanařová, Martina
• Other Authors: Nachtigal, Petr
• Format: Dissertation
• Language: Czech
• Published: 2008
• Online Access: http://www.nusl.cz/ntk/nusl-292586

Atherosclerosis is a chronic inflammatory disease of blood vessels with a successive, very slow progress which takes many years and ,,plugs"disabled vessels. Faults and changes in representation and quantity of lipoproteins are for this disease very typical. In human medicine is mostly used medication Statins during a therapy of dyslipoproteinemia. The aim is this thesis was to describe the changes of biochemical lipoproteins levels at the various mice models of atherosclerosis. Furthermore we wanted to describe the changes lipids after administering of atorvastatin in apoE-defitient mice and apoE/LDLr-deficient mice. Female of apoE-deficient mice and apoE/LDLr-deficient mice were divided into a control and with atorvastatin treated group, where atorvastatin was administered in a period of 8 weeks in a dose of 100 mg/kg mouse. Biochemical analysis of lipids was performed by a separation method of lipoproteins by means of ultracentrifugal technique. Biochemical analysis of blood of apoE-deficient mice demonstrated, that 8 weeks atorvastatin treatment in a dose of 100 mg/kg significantly increased levels of total cholesterol, VLDL, LDL and HDL cholesterol. The blood analysis of apoE/LDLr-deficient mice demonstrated, that 8 weeks long treatment with atorvastatin in the same doses significantly reduced...